#BALU CARTOON SERIES#

Originally intended to span 40 episodes (ending after the Baby Saga), the series continued for another 24 episodes, and is concluded by the TV special Dragon Ball GT: A Hero's Legacy. The Dragon Ball GT series is the shortest of the Dragon Ball series, consisting of only 64 episodes as opposed to its predecessor, Dragon Ball Z, which consisted of 291 episodes, Dragon Ball, which consisted of 153, and its successor series Dragon Ball Super, with 131 episodes. These BAL cells can then be used for FACS analysis, spun onto slides for staining ( Figure 2), and/or lysed for RNA or protein extraction.Dragon Ball GT РіС“вЂ°РіС“В©РівЂљТ‘РіС“С–РіС“СљРіС“СРіС“В« РїСВ§РїСТ‘ РівЂљС‘РіС“СРіС“вЂ РівЂљР€РіС“С Doragon BР•РЊru JР”В« TР”В«Р”Р„ction, Adventure, Comedy, Fantasy, Science fiction, Martial artsР”Вragon Ball GT ( РіС“вЂ°РіС“В©РівЂљТ‘РіС“С–РіС“СљРіС“СРіС“В« РїСВ§РїСТ‘ РівЂљС‘РіС“СРіС“вЂ РівЂљР€РіС“С, Doragon BР•РЊru JР”В« TР”В«, GT standing for 'Grand Tour', commonly abbreviated as DBGT) is one of two sequels to Dragon Ball Z, whose material is produced only by Toei Animation, and is not adapted from a preexisting manga series. Resuspend BAL cell pellets in 400 Р’Вµl of PBS, count the cells by taking about 20 Р’Вµl of the cell sample to a hemocytometer and counting the cells under a microscope. Resuspend BAL cell pellets in 100-200 Р’Вµl ACK lysis buffer, lyse red blood cells on ice for 10 min, and then add 1 ml of PBS to stop lysis. Resuspend BAL cell pellets in 200 Р’Вµl of PBS and combine the cell suspensions. Pathogens, proteins such as cytokines/chemokines, and other components in the supernatants of BAL fluid (BALF) can be analyzed by bicinchoninic acid protein assay, ELISA, luminex assays, and/or other assays. Transfer the supernatant to a new 5 ml tube, add protease inhibitor cocktail to a final concentration of 1x and PMSF to a final concentration of 1 mM, and mix well. Record BAL volume according to the scales on the 1.5 ml EP tubes.Р”В¬entrifuge at 800 Р“вЂ” g for 10 min at 4 РІВРЉ. Repeat steps 10-11 for additional three times. Slowly inject and aspirate the PBS 4 times, remove the syringe from catheter, and save the recovered lavage fluid in 1.5 ml Eppendorf (EP) tubes on ice. Load a 1 ml syringe with 0.8 ml of PBS and place it in the end of the catheter ( Figure 1H). Insert a 22 G x 1РІР‚Сњ Exel Safelet Catheter into the trachea, remove the stylet hub, and tie the catheter and the trachea together firmly with a nylon string ( Figures 1E-1G). Use forceps to put a ~10 cm-long nylon string under the trachea ( Figure 1C).Р”В¬arefully cut the ribs to expose the heart and the lungs ( Figure 1D).

Gently remove the muscle around the neck to expose trachea ( Figure 1B).

#BALU CARTOON SKIN#

Use scissors to make incision in the skin from abdomen to neck, and tear skin with forceps to expose thoracic cage and neck ( Figure 1A). Phosphate-buffered saline (PBS) (see Recipes) Note: This product has been discontinued.Р”В®thylenediaminetetraacetic acid disodium salt dihydrate (Na 2-EDTA-2H 2O) (Sigma-Aldrich, catalog number: E5134-100G) Potassium bicarbonate (KHCO 3) (Sigma-Aldrich, catalog number: P9144-1KG) Potassium phosphate monobasic (KH 2PO 4) (Acros Organics, catalog number: 205925000)Р”Р„mmonium chloride (NH 4Cl) (Sigma-Aldrich, catalog number: A9434-1KG) Potassium chloride (KCl) (Sigma-Aldrich, catalog number: P9541)Р”Вisodium hydrogen phosphate heptahydrate (Na 2HPO 4.7H 2O) (Fisher Scientific, catalog number: BP331-500) Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S9625) Phenylmethylsulfonyl fluoride (PMSF) (Thermo Fisher Scientific, Thermo Scientific TM, catalog number: 36978) Protease inhibitor cocktail (Roche Diagnostics, catalog number: 11697498001) Nylon string (Dynarex, catalog number: 3243)Р”Сћ2 G x 1РІР‚Сњ Exel Safelet Catheter (Exel International, catalog number: 26746)Р”РЋ ml syringe (BD, catalog number: 309659)Р”РЋ.5 ml Eppendorf tubes (VWR, catalog number: 87003-294)Р”В .22 Р’Вµm filter (EMD Millipore, catalog number: SLGP033RS)Р”В§0% ethanol (Decon Labs, catalog number: 2701)

Needles (BD, catalog number: 305167) or tapes

Balu cartoon